FIGURE 4.

Correlation of rhFcγR3-mediated ADCC and JNL cell responses. (A) An NK cell line expressing rhFcγR3A:02 was incubated with CAM-labeled Raji cells for four hours at a 5:1 E:T ratio in the presence of serial dilutions of anti-CD20 antibodies. Percent-specific lysis was calculated from the release of CAM into the supernatant as measured using a Victor X4 multiplate reader (excitation 485 nm and absorbance 535 nm). Error bars indicate SD of the mean for triplicate measurements at each antibody concentration. (B) Mean EC₅₀ values for ADCC responses were calculated from three independent experiments (Table II) and were compared to mean EC₅₀ values for rhFcγR3A:02⁺ JNL cell responses by two-tailed Pearson correlation.