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. Author manuscript; available in PMC: 2021 Dec 15.
Published in final edited form as: J Immunol. 2020 Nov 18;205(12):3319–3332. doi: 10.4049/jimmunol.2000501

FIGURE 5.

FIGURE 5.

Correlation of ADCC by rhesus macaque PBMC and rhFcγR3-mediated JNL cell responses. (A) JNL cells expressing rhFcγR3A:02 were incubated for six hours with CD20-transduced CEM.NKR-CCR5 at a 1:1 E:T ratio with serial dilutions of anti-CD20 antibodies and luciferase activity was measured using a Victor X4 multiplate reader. (B) Unstimulated PBMCs from two different rhesus macaques (r14094 and r15023) were incubated for four hours with CAM-labeled CD20+ CEM.NKR-CCR5 cells at a 20:1 E:T ratio in the presence of anti-CD20 antibodies. Percent-specific lysis was calculated from the amount of CAM released in the supernatant and error bars indicate SD of the mean for triplicate measurements at each antibody concentration. (C) The relationship between the mean rhFcγR3A:02+ JNL cell responses (EC50) from two independent experiments and the mean area under the curve (AUC) values for ADCC responses was determined by two-tailed Pearson correlation.