Skip to main content
. Author manuscript; available in PMC: 2021 Dec 7.
Published in final edited form as: Dev Cell. 2020 Oct 9;55(5):617–628.e6. doi: 10.1016/j.devcel.2020.09.020

Figure 1. MIA triggers macrophage accumulation at the embryonic ChP and modifies CSF composition.

Figure 1.

(A) Schematic of experimental paradigm and E14.5 brain. (B) LV ChP macrophages from saline- or polyI:C conditions. Arrow: macrophages in ChP stromal space; arrowheads: macrophages at ChP surface. Scale = 20 μm. (C) Iba1+ macrophage numbers per LV ChP. **P = 0.0030, unpaired t-test. (D) Percent of epiplexus ChP macrophages. **P = 0.0029, unpaired t-test. (E) Iba1+ macrophage circularity. ****P < 0.0001, unpaired t-test. Dashed lines: median and quartile values. Data points: individual cells. (F) Schematic depicting major morphological states of Iba1+ macrophages at E14.5. (G) Round ChP Iba1+ macrophages (% of total). **P = 0.0020, unpaired t-test. See also Figure S1F. (H) CD68+ macrophages from MIA E14.5 LV ChP Arrows: “thin” macrophage, CD68−;Iba1+; arrowheads: “round” macrophages, CD68+;Iba1+. Scale = 50 μm. (I) Macrophages (Iba1+) that are phagocytic (CD68+;Iba1+, in %) in (H) (% of total). **P = 0.0021, unpaired t-test. (J) Cytokines and chemokines in E14.5 CSF and AF. n ≥ 4 litters per group. (K) Relative changes in CCL2 in CSF samples from (J). *P = 0.025, unpaired t-test. (L) E14.5 CSF [CCL2] analyzed by sandwich ELISA. n = 3 litters per group, *P = 0.0039, unpaired t-test. All data presented as mean ± S.E.M., except in (J) where mean values are presented. See also Figure S1, Tables S1-S2.