Mice received human IL-38 (3–152) or vehicle 48, 24 and 2 hours prior to intraperitoneal instillation of MSU crystals (1 mg/mouse). Another group of mice received anakinra at 10 mg/kg one hour before MSU crystals.A. Abdominal wall temperature 4 hours after MSU crystals, immediately prior to anesthesia and sacrifice.B. Levels of peritoneal fluid KC.C. Plasma IL-6 and D. plasma KC 4 hours after MSU crystals. Data are presented as mean ±SEM, *p < 0.05, **p < 0.01 compared to vehicle, n=5 mice per group. Differences between vehicle and anakinra or vehicle and IL-38 are indicated above the bar.