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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Neurobiol Dis. 2020 Oct 28;147:105153. doi: 10.1016/j.nbd.2020.105153

Fig. 3.

Fig. 3.

LAR peptide did not affect morphological innervation of the diaphragm by PhMNs. Whole-mount immunohistochemistry of diaphragm was used to label postsynaptic nicotinic acetylcholine receptors with AlexaFluor-conjugated alpha-bungarotoxin (red) and pre-synaptic phrenic motor axons all the way to their terminals with an antibody against neurofilament (SMI-312, green) and a second antibody against synaptic vesicle protein 2 (SV2, also green). As shown in representative confocal z-stacks, nearly all NMJs in ipsilateral hemidiaphragm were intact in C2 hemisection animals receiving either DMSO-only (A-F) or LAR peptide (G-L). As shown in the higher magnification images, intact NMJs were characterized by complete apposition of the pre- and post-synaptic labeling. There were no differences in the percentages of intact, completely-denervated (Denv) or partially-denervated (P.Denv) NMJs, the portion of NMJs innervated by multiply phrenic motor axons (M.A.) or the percentage of NMJs with thin preterminal axons (T.S.) between rats treated with DMSO-only and LAR peptide (M), n = 3 for DMSO-only, n = 4 for LAR peptide. P > 0.05 vs the respective control. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)