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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Gastroenterology. 2020 Sep 4;159(6):2077–2091.e8. doi: 10.1053/j.gastro.2020.08.051

Figure 6. ILC2 depletion blocks M2-macrophage and eosinophil infiltration after gastric damage.

Figure 6.

Immunostained sections from Rat IgG2b (n=4), Anti-CD90.2 (n=4), Rat IgG2b + L635 (n=3), and Anti-CD90.2 + L635 (n=4) treated C57BL/6J mice. (A) Representative images of macrophage/ dendritic cell marker F4/80 (red), alternatively activated macrophage marker CD163 (green), with nuclear counter stain Hoechst (blue) (scale bars = 100 μm).

Magnified inset of F4/80-positive macrophages (right). (B) Quantification of F4/80 and CD163 dual-positive alternatively activated macrophages per 20X objective field. (A) Representative images of immunohistochemical staining for eosinophil specific marker Major Basic Protein (MBP). (B) Quantification of MBP-positive eosinophils per 10X objective field. Statistical significance determined by one-way ANOVA with Bonferroni’s post-hoc multiple comparisons test. **** for p < 0.0001. Error bars represent mean ± SD.