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. 2020 Nov 26;11:595053. doi: 10.3389/fimmu.2020.595053

Figure 2.

Figure 2

IL-33 induces IL-6 expression and secretion in primary AML cells. (A) The relationship between IL-33 and IL-6. Spearman rank correlation analysis was performed to evaluate the correlation of serum IL-33 with IL-6 in patients with AML at diagnosis (n = 10). (B) PBMCs and BMMCs from patients diagnosed with AML were incubated for 72 h with IL-33 (100 ng/ml) or combined with anti-IL-33 (100 ng/ml). CBA was used to simultaneously measure IL-6 in supernatants from cell cultures, and plotted as fold change compared with untreated samples. (C, D) RNA/cDNA expression of cells from (B) was analyzed using quantitative real-time PCR using Actb as a reference control. Data are plotted as relative gene expression compared with the untreated control. A significant difference in the mean IL-6 mRNA expression between compared with untreated cells was found in both BM and PB samples. n≥3; *P < 0.05; **P < 0.01. One-way ANOVA test.