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. 2020 Nov 26;11:595053. doi: 10.3389/fimmu.2020.595053

Figure 3.

Figure 3

IL-33 activates p38 MAPK pathway in primary AML samples. (A, B) The AML cell line HL-60 were treated with IL-33 (100 ng/ml) or in combination with SB (20 µM). P-p38, pATF2, or GAPDH protein expression was probed by Western blot analysis. The bar graph shows the quantification of p-p38 and pATF2 protein in all groups. (C–F) PBMCs and BMMCs from patients diagnosed with AML were incubated for 72 h with IL-33 (100 ng/ml) or in combination with SB (20 µM). P-p38, p38, pATF2, or GAPDH protein expression was probed by Western blot analysis. The bar graphs show the quantification of p-p38 and pATF2 protein in all groups. n≥3; *P < 0.05; **P < 0.05. One-way ANOVA test.