Figure 4.

IL-33 promotes cell survival via p38 MAPK pathway in primary AML samples. BMMCs (A, B) and PBMCs (C, D) from pediatric patients with AML were cultured with IL-33 (100 ng/ml), or SB (20 µM) alone, or in combination for 72 h, and apoptosis was measured by Annexin V staining. Bar graphs show the relative Annexin V staining of leukemia cells as compared to the untreated cells. (E) BMMCs from AML patients were analyzed for cell cycle status. Bar graph shows the relative percentage of leukemia cells in the indicated phase of the cell cycle after culture for 72 h in the presence of IL-33 (100 ng/ml), SB (20 µM) or in combination, compared to the untreated cells. n≥3; *P < 0.05; **P < 0.05; ****P < .0001. One-way ANOVA test.