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. Author manuscript; available in PMC: 2021 Dec 7.
Published in final edited form as: Dev Cell. 2020 Oct 28;55(5):544–557.e6. doi: 10.1016/j.devcel.2020.10.004

Figure 2: Specific activation of PKA in basal, but not luminal cells, impairs ductal outgrowth.

Figure 2:

Schematic of breeding strategy to generate Krt5-PrkacaCαR/fl mice that specifically express activated PKA in basal cells (A). FACS plots (B) and fluorescence imaging of ducts (C) show the extent of GFP-expressing cells within the basal compartment and ducts that have undergone Cre recombination in Krt5-PrkacaCαR/fl mice and Krt5-Prkacafl/fl controls at 8 weeks. Bar, 500μm (D) Fluorescence imaging of a cross-section of a mammary duct of Krt5-mTmG-PrkacaCαR/fl mice to assess histology and extent of tdTomato vs GFP recombination. Bar, 150μm. Schematic of breeding strategy to generate Krt8-PrkacaCαR/fl mice that specifically express activated PKA in luminal cells (E). FACS plots (F) and fluorescence imaging of ducts (G) show the extent of GFP-expressing that have undergone Cre recombination in Krt8-PrkacaCαR/fl mice and Krt8-Prkacafl/fl controls at 8 weeks. Bar, 500μm. (H) Fluorescence imaging of a cross-section of mammary ducts of Krt8-mTmG-PrkacaCαR/fl mice to assess histology and extent of tdTomato vs GFP recombination. Bar, 150μm.