Extended Data Figure 3. Stable vertical transmission of the Oligo-MM¹² consortium.

(a) Total and (b,c) strain-specific 16S DNA quantitative PCR and of fecal samples of mice stably colonized with the Oligo-MM¹² consortium. In (a) ΔCT was calculated in respect to a reference SPF sample, marked by the black filled symbol, to which all other values were compared. In (c), Ct-values were used to quantify the relative abundance of each species (see Methods). LOD, limit of detection. P, parental strain (colonized by gavage). F1, first generation after P. Note that Bifidobacterium animalis (YL2) is usually undetectable in feces, as previously described¹⁹.