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. 2020 Nov 26;8:617585. doi: 10.3389/fbioe.2020.617585

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Copyright © 2020 Wang, Wang, Zhao, Yan, Huang, Yin, Huang, Chen and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro biocompatibility test of Mg-IHC. (A) Magnesium ion concentration released by Mg-IHC in vitro was detected after 3, 7, 14, 21, and 28 days. (B) Cell viability detected by CCK-8 kit on 1, 3, and 7 days, respectively. (C) Statistics of the percentage of dead cells. Compare with Ctrl. **P < 0.01; ***P < 0.001. (D) Live/dead staining for MC3T3-E1 cells. The white arrows indicate dead cells. (E) Cell actin cytoskeleton staining showing the morphology of MC3T3-E1 cells on different substrate, stained with FITC-phalloidin.