FIGURE 3.

The effect of Mg-IHC on osteogenesis was examined in vitro. (A) MC3T3-E1 cells were cultured with GelMA and Mg-IHC extracts, and ALP was stained with ALP staining kit 7 days after osteogenesis induction, and calcium nodules were stained with alizarin red 14 days later. (B) Quantitative results of alizarin red staining. (C) MC3T3-E1 cells were cultured with GelMA and Mg-IHC extracts, and ALP activity was quantitatively detected using ALP activity kit 7 days after osteogenesis induction. (D–F) MC3T3-E1 cells were cultured with GelMA and Mg-IHC extracts, and 14 days after osteogenesis induction, the expression of osteogenesis-related genes COL-I, OCN, and RUNX2 was detected by real-time PCR. *P < 0.05; **P < 0.01; ***P < 0.001.