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Schematic illustration of the (TMT)-based quantitative phosphoproteomic workflow. 5-8F cells treated with suberoylanilide hydroxamic acid for 24 h were subjected to six-plex TMT labeling. Combined labeled peptides were subjected to TiO2 enrichment. The enriched phosphopeptides were analyzed using an Orbitrap-equipped mass spectrometer. TMT, tandem mass tag; LC–MS/MS, liquid chromatography–tandem mass spectrometry; TiO2, titanium dioxide.
