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. 2020 Dec 9;11:6318. doi: 10.1038/s41467-020-20136-w

Fig. 5. PEL SEs are sensitive to THZ1 and JQ1 perturbations.

Fig. 5

a MYC and IRF4 expression in BCBL1 and JSC cells treated with increasing doses of JQ1 and THZ1 or vehicle for 2 days was determined by immune blotting. GAPDH was used as loading control (n = 3, independent experiments with similar results). b BCBL1 H3K27ac ChIP-qPCRs signals at MYC SE, IRF4 SE, and MYC promoter following JQ1 and THZ1 or vehicle treatment for 2 days (n = 3, independent experiments). A two-tailed unpaired t-test was used for statistical analysis. c BCBL1 and JSC cell growth following treatment with different doses of JQ1 or THZ1, DMSO was used as control. Cell growth was measured by luminescent cell viability assay (n = 4, independent experiments). Two-way analysis of variance (ANOVA) followed by Bonferroni multiple comparison analysis was used for analysis. d Graphic model of THZ1 and JQ1 treatment decreased PEL SE H3K27ac signals, which further downregulated SE-linked essential genesʼ (IRF4 and MYC) expression and inhibited cell growth. The error bars indicate the SEM for the averages across the multiple experiments. Source data are provided as a Source Data file.