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. 2020 Dec 9;10:21591. doi: 10.1038/s41598-020-78550-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Representative TEM/EDX analyses of FIB-prepared cell lamellae from single NR8383 cells after 24 h of exposure to 100 µg mL⁻¹ unfunctionalized silica particles. The thickness of each TEM lamella was 100 nm. TEM images of cell lamellae were prepared after incubation with microspheres (a) or microrods (c); white arrows indicate particles inside the cells. Corresponding EDX spectra (b and d) of the elements detected within the regions of interest of the cell lamellae (red boxes in a and c) confirmed the presence of silica.