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. 2020 Nov 26;14:581435. doi: 10.3389/fncir.2020.581435

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Copyright © 2020 Huang, Zhou, Chen, Chen and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of GCaMP6 expression in young adult and old mice. (A) Representative coronal sections of the mouse cortex stained for GFP and MAP2 in 4-month-old wild type (WT), 4- and 23-month-old transgenic Thy1-GCaMP6s line 1 mice. (B) Expression levels of GCaMP6s (F_Anti-GFP/F_Anti-MAP2) in individual L5 pyramidal neurons. (C) Distribution of GCaMP6s levels in individual neurons. Summary data are presented as mean ± SEM. ****P < 0.0001 by Kolmogorov–Smirnov tests.