TABLE 3.

Additional mutations identified in 12 AK38.12 T1 lines grown at each of three different temperatures and assayed for novel mutations at guides targeting downstream of locus 1 (TraesCS7A02G014100) and locus 2 (TraesCS7A02G146100).

Temperature	Location	Guide	Hom	Het	Wt
20°C		guide 1	0	0	0
	Locus 1	guide 2	0	0	0
		guide 3	1	0	11
		guide 4	12	0	0
	Locus 2	guide 5	0	0	12
		guide 6	0	0	12
28°C		guide 1	0	0	12
	Locus 1	guide 2	0	0	12
		guide 3	0	5	7
		guide 4	12	0	0
	Locus 2	guide 5	0	0	12
		guide 6	0	0	12
37°C		guide 1	0	5	7
	Locus 1	guide 2	0	0	12
		guide 3	0	3	9
		guide 4	12	0	0
	Locus 2	guide 5	0	5	7
		guide 6	0	0	12

T0 line AK38.12 contained a homozygous −4 bp edit guide which was inherited by all 12 T1 lines, shown in bold; additional mutations are shown for the other 5 guide targets. Hom, homozygous mutation; het, heterozygous mutation, WT, wild type sequence.