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. 2020 Nov 26;7:582890. doi: 10.3389/fcvm.2020.582890

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Copyright © 2020 Wang, Xie, Jiang, Wu, Xue, Dong, Fan, Dai, Chen, Li, Chen, Ye, Fang, Choy, Zhao, Dong and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hispidulin promoted Sirt1 expression in mouse hearts. (A) Representative immunoblots of the effects of hispidulin on the expression of Sirt1 in mice 1 week after surgery. (B) Quantitative analysis of Sirt1 and GAPDH expression in vivo (n=3). (C) Representative immunoblots of the effects of hispidulin on the expression of Sirt1 in mice 4 weeks after surgery. (D) Quantitative analysis of Sirt1 and GAPDH expression in vivo (n = 5). Data are expressed as the mean ± SE. Significance of the difference between Hispidulin+Sham and Hispidulin+AB: ^*p < 0.05; significance of the difference between DMSO+AB and Hispidulin+AB: ^#p < 0.05. (E) Representative immunoblots of the effects of hispidulin on the expression of Sirt1 in isolated adult mouse cardiomyocytes 24 h after hispidulin treatment. (F) Quantitative analysis of Sirt1 and GAPDH expression in vitro (n = 3). Data are expressed as the mean ± SE. Significance of the difference between DMSO and Hispidulin: ^*p < 0.05 (n represents the number of independent experiments).