Skip to main content
. 2020 Nov 26;7:582890. doi: 10.3389/fcvm.2020.582890

Figure 8.

Figure 8

EX527 blocked the cardioprotective effects of hispidulin on mice. EX527 were injected intraperitoneally 1 day before surgery through 4 weeks after surgery. (A) Images of hearts harvested 4 weeks after surgery. The scale bar refers to 2 mm. (B) H&E staining of heart paraffin sections 4 weeks after surgery. Scale bar refers to 50 μm. (C) Echocardiographic images of the hearts in each group. (D–H) Histograms of LVEDD (D), LEVSD (E), LVPWD (F), EF% (G) and FS% (H) (n = 5–16). (I) The mRNA expression of ANP was detected by qPCR. GAPDH was used as an internal control (n = 4). (J–O) The mRNA expressions of ETC complex subunits including Atp5o (J), Atp5b (K), Usmg5 (L), Atp5j1 (M), Ndufa3 (N) and Ndufa5 (O) were detected by qPCR. GAPDH was used as an internal control (n = 4). Data are expressed as the mean ± SE. Significance of the difference between DMSO+Sham and other 3 groups, respectively: *p < 0.05; significance of the difference between DMSO+AB and Hispidulin+AB: #p < 0.05; significance of the difference between Hispidulin+AB and Hispidulin+AB+EX527: &p < 0.05 (n represents the number of independent experiments).