TABLE 1.
Strains and plasmids used in the current study.
| Strains or plasmids | Relevant characteristic | Source |
| Strains | ||
| Escherichia coli | ||
| DH5α | supE44 ΔlacU169 (φ 80lacZΔM15) hsdR17 recAlendAlgyrA96 thi1 relA | Stratagene (Santa Clara, CA, United States) |
| Saccharomyces cerevisiae | ||
| AY15 | Commercial liquor yeast strain | Tianjin Industrial Microbiology Key Laboratory |
| a8 | MATa, haploid yeast strain from AY15 | Li W. et al., 2017 |
| α5 | MATα, haploid yeast strain from AY15 | Li W. et al., 2017 |
| α5-TB1 | MATα, ΔBDH1:loxP | This study |
| α5-VB1 | MATα, ΔBDH1:loxP-PGK1p-BDH1-PGK1t | This study |
| α5-TB2 | MATα, ΔBDH2:loxP | This study |
| α5-VB2 | MATα, ΔBDH2:loxP-PGK1p-BDH2-PGK1t | This study |
| α5-D1B2 | MATα, ΔBDH1:loxP-PGK1p-BDH2-PGK1t | This study |
| α5-VI2 | MATα, ΔILV2:loxP -PGK1p-ILV2-PGK1t | This study |
| AY-B1 | MATα/MATa, ΔBDH1:loxP/BDH1 | This study |
| AY-SB1 | MATα/MATa, ΔBDH1:loxP/ΔBDH1:loxP | This study |
| AY-D1B2 | MATα/MATa, ΔBDH1:loxP-PGK1p-BDH2-PGK1t/BDH1 | This study |
| AY-SD1B2 | MATα/MATa, ΔBDH1:loxP-PGK1p-BDH2-PGK1t/ΔBDH1:loxP-PGK1p-BDH2-PGK1t | This study |
| Plasmids | ||
| pUG6 | E. coli/S. cerevisiae shuttle vector, containing Amp marker and loxP-KanMX-loxP cassette | Gueldener et al., 2002 |
| Yep352 | URA3 marker, Amp marker ori control vector | Invitrogen (Carlsbad, CA, United States) |
| pSH-Zeocin | Zeo marker, Cre expression vector | Li et al., 2017 |
| YEPI2KPB1 | Amp marker, Kan marker, containing ILV2A-loxP-KanMX-loxP−PGK1p-BDH1-PGK1t-ILV2B | Shi et al., 2016 |
| Yep-KPB2 | Amp marker, Kan marker, containing loxP-KanMX-loxP-PGK1p−BDH2-PGK1t | Shi et al., 2017 |