TABLE 3.
Summary of the results and calculated yields for the serum flask cultivations of strains B. subtilis MG1 (PnarG-lacZ) and MG5 (PnasD-lacZ) employing 7.5 g/L and 10 g/L glucose and different ammonium concentrations.
| Strain | 7.5 g/L glucose | 10 g/L glucose | |||||
| 0.001 mol/L NH4+ | 0.1 mol/L NH4+ | 0.2 mol/L NH4+ | 0.001 mol/L NH4+ | 0.1 mol/L NH4+ | 0.2 mol/L NH4+ | ||
| CDWmax [g/L] | MG1 | 0.2990.033 | 0.3190.013 | 0.4850.033 | 0.4590.007 | 0.3790.007 | 0.4980.033 |
| MG5 | 0.3990.040 | 0.3920.007 | 0.4650.027 | 0.3320.093 | 0.4050.020 | 0.4250.040 | |
| Overall μ [1/h] | MG1 | 0.0500.004 | 0.0610.001 | 0.0760.004 | 0.0440.008 | 0.0340.002 | 0.0420.002 |
| MG5 | 0.0590.002 | 0.0670.002 | 0.0620.003 | 0.0550.002 | 0.0450.001 | 0.0510.009 | |
| YX/S [g/g] | MG1 | 0.0400.001 | 0.0440.004 | 0.0780.012 | 0.0370.003 | 0.0290.001 | 0.0400.003 |
| MG5 | 0.0560.004 | 0.0810.001 | 0.0580.003 | 0.0480.004 | 0.0360.002 | 0.0520.008 | |
| Surfactin [mg/L] | MG1 | 109.973.23 | 94.552.00 | 83.197.37 | 156.203.46 | 136.404.40 | 87.353.99 |
| MG5 | 85.5313.41 | 85.613.97 | 48.492.02 | 74.9017.95 | 66.4215.35 | 32.637.82 | |
| maximum Miller Units | MG1 | 238.373.08 | 156.518.88 | 115.902.90 | 231.709.16 | 152.0725.33 | 119.458.24 |
| MG5 | 669.2935.62 | 692.4844.51 | 666.4616.74 | 779.2420.70 | 982.14196.30 | 708.5678.74 | |
| Miller Units at CDWmax | MG1 | 176.5730.27 | 145.768.51 | 77.120.45 | 127.070.95 | 116.231.85 | 76.214.02 |
| MG5 | 605.2813.14 | 543.5238.63 | 569.5847.52 | 740.50173.45 | 746.76136.21 | 559.6370.19 | |