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. 2020 Nov 26;8:588050. doi: 10.3389/fcell.2020.588050

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Copyright © 2020 Wang, Liu, Liu, Li, Wan, Yang, Su, Cheng, Liu, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of retinal pigment epithelium (RPE) cells. (A) The morphology of RPE cells from passage 0 by phase contrast microscopy. Scale bar, 100 μm. (B) The morphology of RPE cells from passage 4 by phase contrast microscopy. Scale bar, 100 μm. (C) Western blots of RPE65 in RPE cells. β-Actin served as the internal control. (D) Immunofluorescence assays of RPE65 in RPE cells. Scale bar, 50 μm. (E) Western blots of PDGFRβ in RPE cells. NIH/3T3 cell was used as the positive control. β-Actin served as the internal control. (F) Western blots of CD31 in RPE cells. Human umbilical vein endothelial cell (HUVEC) was used as the positive control. β-Actin served as the internal control.