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. 2020 Nov 26;8:588050. doi: 10.3389/fcell.2020.588050

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Copyright © 2020 Wang, Liu, Liu, Li, Wan, Yang, Su, Cheng, Liu, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The cocultured ESCs upregulated PI3K pathway-related markers of replicative senescent RPE cells. (A) Expression of PI3K pathway-related markers in the RR and RRE groups, as assessed by RT-qPCR (n ≥ 3 biological repeats). (B) Results from PI3K pathway-related markers in the RR and RRE groups as determined by western blotting. β-Actin was used as the internal reference. (C) The expression levels of PI3K pathway-related markers in the RR and RRE groups as determined by immunofluorescent staining. Scale bar, 50 μm. Data are presented as the mean ± SD. ^∗P < 0.05; ^****P < 0.0001.