FIGURE 11.

Inhibition of the TGFβ pathway upregulated senescence-related positive markers and downregulated senescence-related negative markers in ESCs-cocultured premature senescent RPE cells. (A) Expression of the TGFβ pathway and cellular senescence-related markers in the PRHE and PRHE-SB groups, as assessed by RT-qPCR (n ≥ 3 biological repeats). (B) Results of the TGFβ pathway and cellular senescence-related markers in the PRHE and PRHE-SB groups as determined by western blotting. β-Actin was used as the internal reference. (C) The expression levels of the TGFβ pathway and cellular senescence-related markers in the PRHE and PRHE-SB groups as determined by immunofluorescent staining. Scale bar, 50 μm. Data are presented as the mean ± SD. ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001; ^****P < 0.0001. PRHE-SB: RPE cells from passages 4 to 6 treated with 400 μM H₂O₂ and then cocultured with ESCs with 10 μM SB431542.