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. 2020 Nov 26;8:588050. doi: 10.3389/fcell.2020.588050

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Copyright © 2020 Wang, Liu, Liu, Li, Wan, Yang, Su, Cheng, Liu, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of the PI3K pathway upregulated senescence-related positive markers and downregulated senescence-related negative markers in ESCs-cocultured replicative senescent RPE cells. (A) Expression of the PI3K pathway and cellular senescence-related markers in the RRE and RRE-LY groups, as assessed by RT-qPCR (n ≥ 3 biological repeats). (B) Results for the PI3K pathway and cellular senescence-related markers in the RRE and RRE-LY groups as determined by western blotting. β-Actin was used as the internal reference. (C) The expression levels of the PI3K pathway and cellular senescence-related markers in the RRE and RRE-LY groups as determined by immunofluorescent staining. Scale bar, 50 μm. Data are presented as the mean ± SD. ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001; ^****P < 0.0001. RRE-LY: RPE cells from passages 8 to 10 cocultured with ESCs and treated with 10 μM LY294002.