FIGURE 13.

Inhibition of the TGFβ and PI3K pathways decreased the proliferative capacities and cell cycle transitions of ESCs-cocultured premature and replicative senescent RPE cells. (A) Proliferation of the PRHE and PRHE-SB groups, as assessed by a CCK-8 proliferation assay (n = 3 biological repeats). (B) Proliferation of the RRE and RRE-LY groups, as assessed by a CCK-8 proliferation assay (n = 3 biological repeats). (C) Proportion of cell cycle distribution in the PRHE and PRHE-SB groups, as assessed by flow cytometry (n = 3 biological repeats). (D) Proportion of cell cycle distribution in the RRE and RRE-LY groups, as assessed by flow cytometry (n = 4 biological repeats). Data are presented as the mean ± SD. ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001; ^****P < 0.0001.