FIGURE 2.

Establishment of RPE premature and replicative senescence cellular models. (A) Senescence-associated galactosidase (SA-β-GAL) activity in RPE cells treated with different concentrations of hydrogen peroxide (H₂O₂), as indicated by phase contrast microscopy. Scale bar, 100 μm. (B) Results from the quantification of SA-β-GAL⁺ cells shown in (A). SA-β-GAL⁺ cells in 4 random fields were scored (n = 3 biological repeats). The results are expressed as the percentage of stained cells. (C) Proliferation of RPE cells treated with different concentrations of H₂O₂, as assessed by a Cell Counting Kit 8 (CCK-8) proliferation assay (n = 6 biological repeats). (D) SA-β-GAL activity in RPE cells from passages 4 to 10, as indicated by phase contrast microscopy. Scale bar, 100 μm. (E) Results from the quantification of SA-β-GAL⁺ cells shown in D. SA-β-GAL⁺ cells in 3 random fields were scored (n = 3 biological repeats). The results are expressed as the percentage of stained cells. Data are presented as the mean ± SD. ^∗∗P < 0.01; ^∗∗∗P < 0.001; ^****P < 0.0001.