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. 2020 Nov 26;8:588050. doi: 10.3389/fcell.2020.588050

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Copyright © 2020 Wang, Liu, Liu, Li, Wan, Yang, Su, Cheng, Liu, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The cocultured embryonic stem cells (ESCs) increased the proliferative capacity of premature and replicative senescent RPE cells. (A) Proliferation of the PR, PRH, and PRHE groups, as assessed by a CCK-8 proliferation assay (n = 3 biological repeats). (B) Proliferation of the RR and RRE groups, as assessed by a CCK-8 proliferation assay (n = 3 biological repeats). Data are presented as the mean ± SD. ^∗∗P < 0.01; ^****P < 0.0001. PR: RPE cells of the control group from passages 4 to 6; PRH: RPE cells from passages 4 to 6 treated with 400 μM H₂O₂; PRHE: RPE cells from passages 4 to 6 treated with 400 μM H₂O₂ and then cocultured with ESCs. RR: RPE cells of the control group from passages 8 to 10; RRE: RPE cells from passages 8 to 10 cocultured with ESCs.