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. 2020 Nov 26;8:588050. doi: 10.3389/fcell.2020.588050

FIGURE 5.

FIGURE 5

The cocultured ESCs reduced the senescent phenotypes of premature and replicative senescent RPE cells. (A) SA-β-GAL activity in the PR, PRH, and PRHE groups, as indicated by phase contrast microscopy. Scale bar, 100 μm. (B) SA-β-GAL activity in the RR and RRE groups, as indicated by phase contrast microscopy. Scale bar, 100 μm. (C,D) Results from the quantification of SA-β-GAL+ cells shown in (A,B), respectively (n = 3 biological repeats). SA-β-GAL+ cells in 4 random fields were scored. The results are expressed as the percentage of stained cells. (E) Reactive oxygen species (ROS) levels in the PR, PRH, and PRHE groups, as assessed by flow cytometry (n = 3 biological repeats). (F) ROS levels in the RR and RRE groups, as assessed by flow cytometry (n = 3 biological repeats). (G,H) Results from the mean fluorescence intensity shown in (E,F), respectively. (I) Mitochondrial membrane potential (MMP) levels in the PR, PRH, and PRHE groups, as assessed by a microplate reader (n = 3 biological repeats). (J) MMP levels in the RR and RRE groups, as assessed by a microplate reader (n = 3 biological repeats). Data are presented as the mean ± SD. ∗P < 0.05; ∗∗∗P < 0.001; ****P < 0.0001.