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. 2020 Nov 26;8:588050. doi: 10.3389/fcell.2020.588050

FIGURE 6.

FIGURE 6

The cocultured ESCs downregulated senescence-related positive markers and upregulated senescence-related negative markers in premature senescent RPE cells. (A) Expression of cellular senescence-related markers in the PR, PRH, and PRHE groups, as assessed by RT- qPCR (n ≥ 3 biological repeats). (B) Results for cellular senescence-related markers in the PR, PRH, and PRHE groups as determined by western blotting. β-Actin was used as the internal reference. (C) The expression levels of cellular senescence-related markers in the PR, PRH, and PRHE groups as determined by immunofluorescent staining. Scale bar, 50 μm. Data are presented as the mean ± SD. P < 0.05; ****P < 0.0001.