TABLE 1.
Genes | Primer sequences |
p53 | Forward: 5′-GGTCCAGATGAAGCTCCCAGA-3′ Reverse: 5′-AGGGACAGAAGATGACAGGGG-3′ |
p21WAF1/CIP1 | Forward: 5′-GTCTTGTACCCTTGTGCCTCG-3′ Reverse: 5′-GCGGATTAGGGCTTCCTCTTG-3′ |
p16INK4a | Forward: 5′-GTGGGTTTGTAGAAGCAGGCA-3′ Reverse: 5′-ATCCCCAGGCATCTTTTGCAC-3′ |
Cyclin A2 | Forward: 5′-TGCTGGAGCTGCCTTTCATTT-3′ Reverse: 5′-GCTGTGGTGCTTTGAGGTAGG-3′ |
Cyclin B1 | Forward: 5′-GATCGCCCTGGAAACGCAT-3′ Reverse: 5′-CACTGCTCCCTCCTTATTGGC-3′ |
Cyclin D1 | Forward: 5′-GCTGTGCATCTACACCGACAA-3′ Reverse: 5′-ATGAAATCGTGCGGGGTCATT-3′ |
TGFB1 | Forward: 5′-TCCGTGGGATACTGAGACACC-3′ Reverse: 5′-TCTCCCGGCAAAAGGTAGGAG-3′ |
SMAD3 | Forward: 5′-CAAGTATGGTAGGGGAGGGCA-3′ Reverse: 5′-TGGGTTTGCTCGTGTGTTTCA-3′ |
ID1 | Forward: 5′-GTTACTCACGCCTCAAGGAGC-3′ Reverse: 5′-ATGTAGTCGATGACGTGCTGG-3′ |
ID3 | Forward: 5′-GAGAGGCACTCAGCTTAGCC-3′ Reverse: 5′-TCCTTTTGTCGTTGGAGATGAC-3′ |
PIK3CG | Forward: 5′-AACACCGACCTCACAGTTTTT-3′ Reverse: 5′-CTCAAGCCACACATTCCACAG-3′ |
PDK1 | Forward: 5′-AGAGGGTTACGGGACAGATGC-3′ Reverse: 5′-GTCTTTGGGTTCTCTGCTGGG-3′ |
PLK1 | Forward: 5′-GGACTGGCAACCAAAGTCGAA-3′ Reverse: 5′-CACCTCGAAACTGTGCCCTTT-3′ |
GAPDH | Forward: 5′-AAAATCAAGTGGGGCGATGCT-3′ Reverse: 5′-GGTTCACACCCATGACGAACA-3′ |