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. 2020 Nov 26;8:588050. doi: 10.3389/fcell.2020.588050

TABLE 1.

Primer sequences for RT-PCR analysis.

Genes Primer sequences
p53 Forward: 5′-GGTCCAGATGAAGCTCCCAGA-3′ Reverse: 5′-AGGGACAGAAGATGACAGGGG-3′
p21WAF1/CIP1 Forward: 5′-GTCTTGTACCCTTGTGCCTCG-3′ Reverse: 5′-GCGGATTAGGGCTTCCTCTTG-3′
p16INK4a Forward: 5′-GTGGGTTTGTAGAAGCAGGCA-3′ Reverse: 5′-ATCCCCAGGCATCTTTTGCAC-3′
Cyclin A2 Forward: 5′-TGCTGGAGCTGCCTTTCATTT-3′ Reverse: 5′-GCTGTGGTGCTTTGAGGTAGG-3′
Cyclin B1 Forward: 5′-GATCGCCCTGGAAACGCAT-3′ Reverse: 5′-CACTGCTCCCTCCTTATTGGC-3′
Cyclin D1 Forward: 5′-GCTGTGCATCTACACCGACAA-3′ Reverse: 5′-ATGAAATCGTGCGGGGTCATT-3′
TGFB1 Forward: 5′-TCCGTGGGATACTGAGACACC-3′ Reverse: 5′-TCTCCCGGCAAAAGGTAGGAG-3′
SMAD3 Forward: 5′-CAAGTATGGTAGGGGAGGGCA-3′ Reverse: 5′-TGGGTTTGCTCGTGTGTTTCA-3′
ID1 Forward: 5′-GTTACTCACGCCTCAAGGAGC-3′ Reverse: 5′-ATGTAGTCGATGACGTGCTGG-3′
ID3 Forward: 5′-GAGAGGCACTCAGCTTAGCC-3′ Reverse: 5′-TCCTTTTGTCGTTGGAGATGAC-3′
PIK3CG Forward: 5′-AACACCGACCTCACAGTTTTT-3′ Reverse: 5′-CTCAAGCCACACATTCCACAG-3′
PDK1 Forward: 5′-AGAGGGTTACGGGACAGATGC-3′ Reverse: 5′-GTCTTTGGGTTCTCTGCTGGG-3′
PLK1 Forward: 5′-GGACTGGCAACCAAAGTCGAA-3′ Reverse: 5′-CACCTCGAAACTGTGCCCTTT-3′
GAPDH Forward: 5′-AAAATCAAGTGGGGCGATGCT-3′ Reverse: 5′-GGTTCACACCCATGACGAACA-3′