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. 2020 Nov 26;8:599890. doi: 10.3389/fcell.2020.599890

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Copyright © 2020 Li and Niswander.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TMEM132A and WLS interact with each other. (A) Schematic representation of FL and truncated constructs of T132A-Flag and WLS-HA. (B) Co-IP of endogenous WLS by FL T132A-Flag (left), and co-IP of endogenous TMEM132A by FL WLS-HA (right) in HEK 293T cells. (C) Co-IP between FL T132A-Flag and FL WLS-HA in HEK 293T cells. (D) Co-IP of WLS-HA by FL, but not SN or C expression constructs lacking TM domain of T132A-Flag in HEK 293T cells. (E) Co-IP of WLS-HA by FL, SNT and STC of T132A-Flag in HEK 293T cells. (F) Co-IP of WLS-HA by FL and ST of T132A-Flag but not by the SNC construct lacking TM domain of T132A-Flag in HEK 293T cells. (G) Co-IP showing strong interaction of FL T132A-Flag with FL and truncation 1-264 of WLS-HA, and weak interaction with truncations 332–428 and 429–541 of WLS-HA in HEK 293T cells. * indicates non-specific band.