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. 2020 Nov 26;8:587693. doi: 10.3389/fcell.2020.587693

FIGURE 3.

FIGURE 3

Inhibiting miR-374b-5p alleviated the renal injury and suppressed M1 macrophage activation. The mice were transfected with miR-374b-5p inhibitor and associated control for 48 h before RIR operation. One day after RIR operation, the serum and kidneys were separated. (A) Serum levels of SCr and BUN in RIR-injured mice with miR-374b-5p inhibitor or NC-inhibitor transfection were determined. (B) Representative PAS staining images of kidneys in mice with or without miR-374b-5p inhibitor treatment. Bar = 50 μm. (C) Representative IHC staining images of CD86 from RIR-injured mice after miR-374b-5p inhibitor administration. Bar = 50 μm. (D,E) qRT-PCR analysis of MCP-1, TNF-α, IL-1β, CD86, and iNOS in the kidneys of RIR-injured mice with different treatments. (F) The quantitative analysis of CD86 positive cells according to flow cytometry results. Data presented as means ± SD for groups of three mice. *p < 0.05, **p < 0.01, ***p < 0.001 vs. the NC-inhibitor group. NC-inhibitor, miRNA inhibitor control.