Skip to main content
. 2020 Nov 26;8:587693. doi: 10.3389/fcell.2020.587693

FIGURE 5.

FIGURE 5

Uptake of exosomes-hypoxia by macrophage promoted M1 macrophage activation. Exosomes were isolated from the TECs, which were exposed to hypoxic or normoxic conditions for 48 h and then were applied to treat naive macrophages at different concentrations (15 or 30 ng/mL) for 48 h. (A) PKH67-labeled exosomes uptake by macrophages. Bar = 50 μm. (B) The expression level of miR-374b-5p in macrophages was detected by qRT-PCR. (C) qRT-PCR analysis of TNF-α, CD86, and iNOS in the macrophages with different treatments. (D) Representative images of CD86 in recipient macrophages were detected by IF. Bar = 50 μm. N = 3, data presented as means ± SD. *p < 0.05 vs. the PBS group. #p < 0.05, ##p < 0.01 vs. the Exo-normoxia group. Exo-normoxia, exosomes derived from the TECs under the normoxic condition for 48 h. Exo-hypoxia, exosomes derived from the TECs, which were exposed to hypoxia for 48 h.