Skip to main content
. 2020 Nov 26;8:587693. doi: 10.3389/fcell.2020.587693

FIGURE 9.

FIGURE 9

Inhibition of exosomal miR-374b-5p alleviated RIRI in mice. The TECs were transfected with miR-374b-5p inhibitor or related control, followed by exposed to hypoxic conditions for 48 h. Then the exosomes were isolated and injected into the mice. The mice were then subjected to RIRI, and the kidney tissues and serum were collected on day 1 after the operation. (A) Representative PAS images of kidneys at 48 h after different treatments. Bar = 50 μm. (B) IHC assessed the expression of CD86 in the kidney from mice with different treatments. Bar = 50 μm. (C) Serum levels of SCr and BUN in RIR-injured mice with exosomes-ctrl (Hypo) or exosomes-miR-374b-5p inhibitor (Hypo) treatment were performed. (D) mRNA expression of MCP-1, TNF-α, IL-1β, CD86, and iNOS in the kidney injected with exosomes-ctrl (Hypo) or exosome-miR-374b-5p inhibitor (Hypo) was detected by qRT-PCR. (E) The proportion of CD86-positive cells was determined by flow cytometry. Data presented as means ± SD for groups of three mice. *p < 0.05, **p < 0.01 vs. the Exo-ctrl (Hypo) group. Exo-ctrl (Hypo), exosomes derived from the hypoxic TECs after NC-inhibitor transfection. Exo-miR-374b-5p inhibitor (Hypo), exosomes derived from the hypoxic TECs after miR-374b-5p inhibitor transfection.