FIGURE 2.

PM₁₀ treatment delays anaphase transition timing during mouse oocyte maturation. (A) Time-lapse microscopy of maturing control (0 mg/mL) and PM₁₀ (5 mg/mL)-treated oocytes. Cyclin B, green; DNA, red. The full time lapse recording can be seen in Supplementary Movie 1 and 2. Scale bar = 20 μm. (B) The fluorescence intensity of cyclin B-GFP normalized at maturation of oocytes undergoing meiotic division. Data are presented as the mean ± SEM. Experimental replicate three times. Sample sizes (n) are indicated. (C) Western blotting of cyclin B and Cdk1 (MPF subunits) in ATI stage oocytes. Oocytes were sampled at 9 h after maturation. (D) Relative protein expression levels of cyclin B and CDK1. Cyclin B protein was degraded at the ATI stage in control oocytes, but was maintained in the PM₁₀ group. The experiment was performed three times. *P = 0.0184. N.S., not statistically significant (P > 0.05).