FIGURE 3.

PM₁₀ exposure induces defects in spindle assembly and chromosome alignment in mouse oocytes. (A) Time-lapse microscopy of maturing control and PM₁₀-treated oocytes. The Spindle, green fluorescence. Yellow arrows indicate misaligned chromosome. (B) Defective spindle morphology and chromosome alignment are shown. Oocytes were fixed at 8 h after meiotic resumption. Green; spindle, and DNA was counterstained using Hoechst 33342 (blue). (C) The percentage of abnormal spindle formation. (D) Quantification of the distance between chromosomes. (E) Localization of pericentrin 8 h after meiotic resumption. Yellow arrows indicate scattered pericentrin. (F) Quantification of the abnormal rate of mislocalized pericentrin. Experimental replicate (N) and n sample size are indicated. ***P < 0.001. Scale bars = 20 μm.