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. 2020 Nov 26;10:564614. doi: 10.3389/fonc.2020.564614

Figure 2.

Figure 2

FMNL1 is regulated by GATA3 in ccRCC cells. (A) The expression of FMNL1 was determined in breast cancer MDA-MB-231 cells. Data were from a GEO study (no. GDS4080). (B) 293T cells were transfected with GATA3. The mRNA expression of FMNL1 was examined. Data were from another GEO study (no. GDS892). (C) RCC10 and A498 cells were transfected with GATA3 overexpression vectors for 36 h. The expression of FMNL1 mRNA was examined by qRT-PCR. *P < 0.05. (D) FMNL1 mRNA levels in 786-O cells transfected with GATA3 siRNAs for 36 h were measured. *P < 0.05. (E) The protein expression of GATA3 and FMNL1 in ccRCC cells with GATA3 overexpression or knockdown was examined by western blot. (F) Dual luciferase reporter assays were performed to indicate the effect of GATA3 on the activity of FMNL1 promoter. **P < 0.01, ***P < 0.001. (G) ChIP assays were used to detect the enrichment of GATA3 on FMNL1 promoter. The binding of GATA3 onto ECHS1 promoter was used as positive control (PC). (H) GATA3 expression was further examined in 306 ccRCC paraffin-embedded tissues. A ccRCC case with low expression of FMNL1 showed high GATA3 expression. (I) The correlation of GATA3 and FMNL1 expression was determined by Pearson correlation analyses in 306 ccRCC cases.