Figure 3.

FMNL1 promotes ccRCC cell migration in vitro and tumor metastasis in vivo. (A) RCC10 and A498 cells were transfected with FMNL1 overexpression vector and selected by G418 for 2 weeks. The mRNA and protein expressions of FMNL1 in stable cells were examined by qRT-PCR and western blot. Stable 786-O cells with FMNL1 knockdown was also established. *P < 0.05. (B, C) Stable cells were seeded onto the transwell chamber with or without Matrigel coating and cultured for 36 h. The migrated or invaded cells were stained by 0.05% of crystal violet, and counted under a microscope the indicate the effect of FMNL1 overexpression (B) or silencing (C) on ccRCC cell migration. *P < 0.05. (D) Stable cells were injected into BALB/c nude mice through tail vein. After 40 days, lungs were dissected. HE staining were performed to show the metastatic nodules in the lungs. The number of tumors in lungs was shown by histogram. *P < 0.05.