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. 2020 Nov 26;10:564614. doi: 10.3389/fonc.2020.564614

Figure 5.

Figure 5

FMNL1 upregulates CXCR2 via HDAC1 in ccRCC cells. (A, B) The expression of CXCR2 mRNA in stable cells with FMNL1 overexpression (A) and knockdown (B) was determined by qRT-PCR. *P < 0.05. (C, D) CXCR2 mRNA expression was determined in cells treated with siRNA for HDAC1, HDAC2 or HDAC3 in human cancer cells. Data were from GEO datasets (no. GDS2294 and GDS4813). (E, F) Cells with FMNL1 overexpression were transfected with HDAC1 siRNAs for 36 h. CXCR2 mRNA (E) and protein (F) expressions were determined by qRT-PCR and western blot. *P < 0.05. (G) The histone H3 acetylation at lysine 9 was determined by ChIP assays in RCC10 cells with transfection of HDAC1 siRNAs. (H) CXCR2 expression was further examined in 306 ccRCC paraffin-embedded tissues. Representative IHC images showed positive correlation of CXCR2 and FMNL1 expression. The correlation of CXCR2 and FMNL1 expression was determined by Pearson correlation analyses in 306 ccRCC cases. (I, J) The effect of CXCR2 expression (I), as well as its combination with FMNL1 expression (J), on the overall survival time of ccRCC patients in SYSUCC cohort was determined. F, FMNL1; C, CXCR2.