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. 2020 Nov 26;11:595733. doi: 10.3389/fgene.2020.595733

FIGURE 7.

FIGURE 7

RNF183 controls ERα expression in endometrial cancer. (A) Ishikawa cells transfected with siRNF183 or siNC and RNF183 knockdown efficiency were examined by RT-PCR. (B) ESR1 mRNA level decreased in the Ishikawa cell line after transfection with siRNF183. (C) The protein level of ERα was downregulated based on RNF183 deletion. (D) RNF183 deletion decreased ERα-dependent expression of the ERE-luciferase activity. (E) The ERE-Luciferase activity was evaluated in Ishikawa cells with overexpression of pcDNA4-myc/his-RNF183 or pcDNA4-myc/his vector or truncated RNF183 without E3 ubiquitin ligase activity (13–60 amino acids). (F) Diminished E2 induced reduction of ERα target genes following inhibition of RNF183 with siRNA. (G) RNF183 positively associated with TFF1, PGR, FOXA1 and XBP1 from the TIMER database. Experiments were repeated in triplicates. Mean ± S.D. (n = 3). **P<0.01, ***P<0.001.