Figure 2.

Model simulations vs. experimental measurements for σ₇₀ induced deGFP expression. (A): Simulated and measured deGFP mRNA concentration vs. time using the small circuit G₂₀ ensemble (N = 140). (B): Simulated and measured deGFP protein concentration vs. time using the small circuit G₂₀ ensemble (N = 140). (C): Global sensitivity analysis of the P70-deGFP circuit parameters. Morris sensitivity coefficients were calculated for the unknown model parameters, where the range for each parameter was established from the ensemble. Uncertainty: Simulations and uncertainty quantification are shown for the generation 20 (G₂₀) ensemble which yielded N = 140 parameter sets that were rank two or below. The parameter ensemble was used to calculate the mean (dashed line) and the 95% confidence estimate of the simulation (gray region). Additionally, the 99% confidence estimate of the mean simulation is shown in orange. Individual parameter set trajectories are shown in blue. Points denote the mean experimental measurement while error bars denote the 95% confidence estimate of the experimental mean computed from at least three replicates.