Table 1.

Characteristic parameters for TX/TL model equations.

Description	Parameter	Value	Units	Reference
RNA polymerase concentration	RX,T	0.06–0.075	μM	a
Ribosome concentration	RL,T	<2.3	μM	a,b
σ70 concentration	σ70	<35	nM	a
initial σ28 concentration	σ28	<20	nM	a
Transcription elongation rate	${\stackrel{.}{v}}_X$	12–30	nt/s	a,d
Translation elongation rate	${\stackrel{.}{v}}_L$	1–2	aa/s	a,b
Transcription saturation coefficient	KX	0.036	μM	i
Polysome amplification constant	KP	10.0	constant	e
Transcription initiation time	$k_I^X$	22	s	i
Translation initiation time	$k_I^L$	1.5	s	e
Default mRNA degradation coefficient	θm	3.75	h-1	a
Default protein degradation coefficient	θp	0.462–1.89	h-1	f,g
Gene concentration σ28		1.5	nM	c
Gene concentration cI-ssrA		1.0	nM	c
Gene concentration deGFP-ssrA		8.0	nM	c
Gene length σ28		811	nt	h
Gene length cI-ssrA		850	nt	h
Gene length deGFP-ssrA		782	nt	h
Protein length σ28		240	aa	h
Protein length cI-ssrA		248	aa	h
Protein length deGFP-ssrA		237	aa	h

Key to references used in the table: (a) Garamella et al. (2016), (b) Underwood et al. (2005), (c) set by experiment, (d) Kassavetis and Chamberlin (1981), (e) estimated in this study, (f) Niederholtmeyer et al. (2015) and Vilkhovoy et al. (2018), (g) Grilly et al. (2007), (h) calculated from plasmid sequence, (i) McClure (1980).