Skip to main content
. 2020 Nov 27;23(12):101864. doi: 10.1016/j.isci.2020.101864

Figure 1.

Figure 1

Antiviral Response to HCMV-UV and SeV-UV in the Presence/Absence of IFN Signaling

(A and B) Wild-type (WT) or IFNAR1 KO THFs were infected with increasing levels of UV-inactivated HCMV before challenging with VSV-GFP 16 hr later. The presence of IFN in the supernatants was assayed by transferring supernatants from infected THFs to naive wild-type THFs and challenging them with VSV-GFP 6 hr later (WT supernatant/IFNAR KO supernatant).

(C and D) WT THFs pretreated with B18R or media alone were infected with increasing levels of HCMV-UV or SeV-UV and challenged with VSV-GFP. Mean fluorescence intensity and standard error from 3 biological replicates was plotted as a percentage of mock infected cells challenged with VSV-GFP. WT and IFNAR KO, WT and IFNAR KO supernatant, or mock and B18R treated THFs were compared by two-way analysis of variance (ANOVA) with Bonferroni post-tests (∗∗ for p < 0.01, ∗∗∗ for p < 0.001).