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. 2020 Nov 27;23(12):101864. doi: 10.1016/j.isci.2020.101864

Figure 2.

Figure 2

ISG Upregulation in Response to HCMV or SeV Particles

(A) Wild-type (WT) or IFNAR1 KO THFs were treated with 84 particles/cell HCMV-UV or 12 particles/cell SeV-UV and RNA harvested for high-throughput RNA sequencing analysis. The 30 most highly upregulated genes across all samples are shown.

(B–D) WT or IFNAR1 KO THFs were treated with 84 or 1,344 particles/cell of HCMV-UV or 12 or 200 particles/cell of SeV-UV and RNA harvested 6 hr post-treatment. Transcript levels of IFIT1 (B), ISG15 (C), and CXCL10 (D) were measured by quantitative RT-PCR. Mean fold change over mock and standard error from 3-6 biological replicates was graphed and WT and IFNAR KO THFs compared by two-way analysis of variance (ANOVA) with Bonferroni post-tests (∗∗ for p < 0.01 and ∗∗∗ for p < 0.001).