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. 2020 Nov 27;23(12):101864. doi: 10.1016/j.isci.2020.101864

Figure 3.

Figure 3

ISG Upregulation in Response to Transfected Nucleic Acid or Replicating Virus

Wild-type (WT) or IFNAR1 KO THFs were transfected with matched sequence dsRNA or dsDNA (A–C) or infected with replication competent HCMV or SeV and RNA harvested 6 hr post-treatment (D–F). Transcript levels of IFIT1 (A and D), ISG15 (B and E), and CXCL10 (C and F) were measured by quantitative RT-PCR. Mean fold change over mock and standard error from 3-7 biological replicates was graphed and WT and IFNAR KO THFs compared by two-way analysis of variance (ANOVA) with Bonferroni post-tests (∗ for p < 0.05, ∗∗ for p < 0.01 and ∗∗∗ for p < 0.001).