Figure 4.

ISG Upregulation in Different Cell Types Infected with HCMV and SeV Particles

(A–L) THFs (A–C), HEL fibroblasts (D–F), NuLi-1 epithelial cells (G–I), or THP-1-derived macrophages (J–L) were infected with 84 particles/cell HCMV or 12 particles/cell SeV in the presence of the protein synthesis inhibitor CHX or DMSO alone and RNA harvested 6 hr post-treatment. Transcript levels of IFIT1, ISG15, and CXCL10 were measured by quantitative RT-PCR. Mean fold change over mock and standard error from 3-4 biological replicates was graphed and DMSO- and CHX-treated cells compared by two-way analysis of variance (ANOVA) with Bonferroni post-tests (∗∗ for p < 0.01 and ∗∗∗ for p < 0.001).