FIGURE 10.

AtPARP2 poly-ADP-ribosylates linear plasmid 2.3 kb DNA fragment; 1 nM 5′-[³²P]-labeled linearized nicked pML2 plasmid DNA was incubated with 2.5–40 nM atPARP2 under the standard reaction conditions. After incubation, the reaction products analyzed by denaturing PAGE. Arrows indicate HMW and LMW PAR–DNA products and free oligonucleotides. For more details, see section “Materials and Methods.”