Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Dependence of the atPARP-catalyzed PAR–DNA formation on reaction conditions; 250 nM atPARP1 and atPARP2 incubated with 20 nM DNA duplex for 30 min at 37°C. (A) atPARP1- and atPARP2-catalyzed DNA PARylation in the presence of varying concentrations of NAD+ (1–1,000 μM). (B) The dependence of DNA PARylation on atPARP protein concentrations (0–250 nM). (C) Time dependence of DNA PARylation by atPARPs (0–30 min). For details, see section “Materials and Methods.”
